17 November 2017:
A new CNBP paper “Statistically strong label-free quantitative identification of native fluorophores in a biological sample,” by Saabah B. Mahbub (first author pictured), Martin Plöschner, Martin E. Gosnell, Ayad G. Anwer and Ewa M. Goldys has just been published in Scientific Reports and is available online.
This work addresses a genuine shortage of methods for real-time continuous monitoring of biochemistry of cells and tissues, especially live cells. Saabah Mahbub and team developed an automated and unbiased unmixing methodology to non-invasively detect the presence and spatial distributions of endogenous fluorophores in retina cells. The method was validated on artificial images, where the addition of a varying known level of noise has allowed to quantify the accuracy of spectral unmixing.
With its capability for high throughput, automation and embedded compatibility with statistical analysis this work will contribute to improved quantification and objectivity in biomedical research.
10 February 2017:
A new publication from CNBP researchers Aziz Ul Rehman (pictured), Ayad Anwer, Martin Gosnell, Saabah Mahbub, Guozhen Liu and Ewa Goldys demonstrates the validation of an innovative hyperspectral unmixing methodology, that can derive chemical information from cell colour.
The work has just been reported in the journal ‘Biomedical Optics Express’ and is accessible online.
Journal: Biomedical Optics Express.
Title: Fluorescence quenching of free and bound NADH in HeLa cells determined by hyperspectral imaging and unmixing of cell autofluorescence.
Authors: Aziz Ul Rehman, Ayad G. Anwer, Martin E. Gosnell, Saabah B. Mahbub, Guozhen Liu, and Ewa M. Goldys.
Abstract: Carbonyl cyanide-p-trifluoro methoxyphenylhydrazone (FCCP) is a well-known mitochondrial uncoupling agent. We examined FCCP-induced fluorescence quenching of reduced nicotinamide adenine dinucleotide / nicotinamide adenine dinucleotide phosphate (NAD(P)H) in solution and in cultured HeLa cells in a wide range of FCCP concentrations from 50 to 1000µM. A non-invasive label-free method of hyperspectral imaging of cell autofluorescence combined with unsupervised unmixing was used to separately isolate the emissions of free and bound NAD(P)H from cell autofluorescence. Hyperspectral image analysis of FCCP-treated HeLa cells confirms that this agent selectively quenches fluorescence of free and bound NAD(P)H in a broad range of concentrations. This is confirmed by the measurements of average NAD/NADH and NADP/NADPH content in cells. FCCP quenching of free NAD(P)H in cells and in solution is found to be similar, but quenching of bound NAD(P)H in cells is attenuated compared to solution quenching possibly due to a contribution from the metabolic and/or antioxidant response in cells. Chemical quenching of NAD(P)H fluorescence by FCCP validates the results of unsupervised unmixing of cell autofluorescence.
14 November 2016:
CNBP PhD student Saabah Mahbub has won best poster prize at the ‘Research by Degrees Showcase’, an event held by the Department of Physics and Astronomy at Macquarie University.
Saabah’s poster was titled ‘Label-free functional characterization of stem cell cartilage system by hyperspectral imaging (with unsupervised unmixing) for applications in regenerative medicine.’
Posters were assessed by external judges that included Mr Hugh Ong (Commonwealth Bank of Australia), Prof Fred Watson (Astronomer-in-Charge, AAO), Mr Steve Frisken (Finisar Corporation and Founder and CEO, Cylite Pty Ltd) and Dr Stephen Thompson (Lecia Microsystems).
Well done Saabah. Great work at showcasing your science!