2017/03/29 : : Lindsay Parker : recent presentation to MQ community : Nima presented to Prof. Barbara Messerle Group; March 9th 2017, 25 minute presentation, Title: Lanthanide Chelates for sensitive time-gated immunodetection.
CNBP Chief Investigator Professor Nicki Packer was an invited chair of a session and leader of the Power Hour (gender equality workshop) at the Gordon Glycobiology conference at Ventura, California 19-24 March, 2017.
As session Chair, Prof Packer led discussion on advances in omics, integrated omics, bioinformatics for glycobiology and the impact of altered glycosylation for human disorders.
The Power Hour was an informal session designed to help address the challenges women face in science and to support the professional growth of women by providing an open forum for discussion and mentoring.
Further conference information is accessible online.
CNBP scientists Chris Ashwood and Prof Nicki Packer at Macquarie University have shown alternative ways to break apart sugars, improving their characterisation in their latest publication in the area of mass spectrometry (Enhancing structural characterisation of glucuronidated O-linked glycans using negative mode ion trap higher energy collision-induced dissociation mass spectrometry).
The work was published online in the journal Rapid Communications in Mass Spectrometry on 9th March 2017 and was funded by the Australian Research Council Centre of Excellence for Nanoscale BioPhotonics.
A new publication from CNBP researchers (lead author Aziz Ul Rehman pictured) reports on the application of hyperspectral imaging in combination with fluorescence spectroscopy and chemical quenching to provide a new methodology to investigate cell metabolism.
The work has just been reported in the journal ‘Biomedical Optics Express’ and is accessible online.
Journal: Biomedical Optics Express.
Title: Fluorescence quenching of free and bound NADH in HeLa cells determined by hyperspectral imaging and unmixing of cell autofluorescence.
Authors: Aziz Ul Rehman, Ayad G. Anwer, Martin E. Gosnell, Saabah B. Mahbub, Guozhen Liu, and Ewa M. Goldys.
Abstract: Carbonyl cyanide-p-trifluoro methoxyphenylhydrazone (FCCP) is a well-known mitochondrial uncoupling agent. We examined FCCP-induced fluorescence quenching of reduced nicotinamide adenine dinucleotide / nicotinamide adenine dinucleotide phosphate (NAD(P)H) in solution and in cultured HeLa cells in a wide range of FCCP concentrations from 50 to 1000µM. A non-invasive label-free method of hyperspectral imaging of cell autofluorescence combined with unsupervised unmixing was used to separately isolate the emissions of free and bound NAD(P)H from cell autofluorescence. Hyperspectral image analysis of FCCP-treated HeLa cells confirms that this agent selectively quenches fluorescence of free and bound NAD(P)H in a broad range of concentrations. This is confirmed by the measurements of average NAD/NADH and NADP/NADPH content in cells. FCCP quenching of free NAD(P)H in cells and in solution is found to be similar, but quenching of bound NAD(P)H in cells is attenuated compared to solution quenching possibly due to a contribution from the metabolic and/or antioxidant response in cells. Chemical quenching of NAD(P)H fluorescence by FCCP validates the results of unsupervised unmixing of cell autofluorescence.
CNBP Research Fellows at Macquarie University, Dr Lindsay Parker (pictured) and Dr Nicole Cordina will attend the 11th International Conference on New Diamond and Nano Carbons 2017 to be held in Cairns, Australia May 28 – June 1.
The conference spans wide research topics from fundamental physical and chemical concepts to applied technologically driven applications with carbon based materials. Those include, but not limited to single crystal diamond, nanodiamonds, carbon nanotubes, graphene and other carbon nanostructures.
Dr Parker has been selected for an oral presentation for her work titled: “Applications of fluorescent nanodiamonds in cellular molecular tracing”.
Dr Cordina has been selected for a poster presentation for her work titled: “Targeting fluorescent nanodiamonds to E-selectin for the detection of inflammation”.