Tag Archives: Ewa Goldys

Cell colour technology shortlisted for Eureka honours


Professor Ewa Goldys, CNBP Deputy Director and Dr Martin Gosnell, Quantitative Pty Ltd, have been selected as finalists in the prestigious Australian Museum Eureka Prizes, for their work in developing technology that enables colour to be used as a uniquely powerful diagnostic tool in medicine.

Selected in the award category ‘2016 ANSTO Eureka Prize for Innovative Use of Technology’, Goldys and Gosnell use modern day microscopes and powerful computer analysis to explore the subtle colour differentiations of cells and tissue, down to a molecular level.

“With our pioneering hyperspectral imaging technique we are able to unveil the biomolecular composition of cells and their nanoscale contents,” said Ewa Goldys, Deputy Director of the ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP) and a Professor at Macquarie University.

“This lets us distinguish between healthy and diseased cells in areas as diverse as embryology, neurodegeneration, cancer and diabetes. Key is the great potential of this technology to impact positively on lives – supporting clinicians in making improved diagnosis and health decisions for patients.”

Noting that it was a pleasure and a privilege to be nominated as a Eureka finalist, Goldys concluded, “Our innovative methodology is letting us probe the very limits of our understanding of life at the molecular level. It’s important that we share these amazing discoveries with the public and the community at large – the Eureka Prizes are the perfect platform to help support us in these efforts.”

Dr Martin Gosnell, CNBP research affiliate and Managing Director at Quantitative Pty Ltd was equally pleased by the Eureka nomination.

“I’m absolutely delighted that our research has been recognised at this level. By using the colour of light from cells and tissues, we are pushing the very frontiers of molecular exploration and measurement.”

“Our high-powered data analysis and imaging expertise is truly opening up new windows into the body.”

The Eureka Prizes are presented by the Australian Museum and reward excellence in research and innovation, science communication and journalism, leadership and school science.

Prize winners will be announced at an Awards Dinner at Sydney Town Hall on Wednesday 31 August 2016.

Site-Dependent Luminescence and Thermal Stability of Eu2+ Doped Fluorophosphate toward White LEDs for Plant Growth

2016/07/21 : : Bronwyn Gibson : Publication : RedXcross16x9 Title – Site-Dependent Luminescence and Thermal Stability of Eu2+ Doped Fluorophosphate toward White LEDs for Plant Growth.

Authors – Jiayu Chen, Niumiao Zhang, Chongfeng Guo, Fengjuan Pan, Xianju Zhou, Hao Suo, Xiaoqi Zhao and Ewa M Goldys.

Abstract – Eu2+ activated fluorophosphate Ba3GdNa(PO4)3F (BGNPF) with blue and red double-color emitting samples were
prepared via a solid-state method in a reductive atmosphere. Their crystal structure and cationic sites were identified in light of X-ray diffraction pattern Rietveld refinement. Three different Ba2+ sites, coordinated by six O atoms referred to as Ba1, two F and five O atoms as Ba2, and two F and six O atoms as Ba3, were partially substituted by Eu2+. Photoluminescence emission (PL) and excitation (PLE) spectra of phosphor BGNPF:Eu2+ along with the lifetimes were characterized at the liquid helium temperature (LHT), which further confirm the existence of three Eu2+ emitting centers resulting in 436, 480, and 640
nm emission from the 5d→4f transitions of Eu2+ in three different Ba2+ crystallographic sites. These emissions overlap with the absorption spectra of carotenoids and chlorophylls from plants, which could directly promote the photosynthesis. Temperature-dependent PL spectra were used to investigate the thermal stability of phosphor, which indicates that the PL intensity of BGNPF:0.9% Eu2+ with optimal composition at 150°C still keeps 60% of its PL intensity at room temperature, in which blue emission has higher thermal-stability than the red emission. Furthermore, the approaching white LED devices have also been manufactured with a 365 nm n-UV LED chip and present phosphor, which make operators more comfortable than that of the plant growth purple emitting LEDs system composed of blue and red light. Results indicate that this phosphor is an attractive dual-responsive candidate phosphor in the application n-UV light-excited white LEDs for plant growth.

Link – http://pubs.acs.org/doi/pdfplus/10.1021/acsami.6b06102.

CNBP contributes to OSA white paper

Ewa Goldys Low Res Edit 015920 June 2016:

CNBP Deputy Director Prof Ewa Goldys, has taken part in the development of an OSA white paper on the subject of label-free techniques for biomedical diagnostics and imaging.

The white paper, just released, was based on the many contributions to an OSA Incubator event held on 16-18 September 2015 at OSA Headquarters, Washington, DC, USA.

The goal of this event was to evaluate the main bottlenecks for clinical translation of label-free optical techniques including technological and regulatory challenges, to identify potential solutions and to develop a prioritized list of recommendations.

Titled, “Label-free Optical Techniques for Biomedical Diagnostics & Imaging: Challenges and Opportunities for Clinical Translation”, the white paper is accessible online.

Ultrasensitive imaging of unique nanoruby probes

Wan Razali6 June 2016:

Our CNBP researchers describe a wide-field time-gated photoluminescence microscopy system, customised for ultrasensitive imaging of unique nanoruby probes in this latest paper published in the Journal of Biophotonics.

Publication Title: Wide-Field Time-Gated Photoluminescence Microscopy for Fast Ultrahigh-Sensitivity Imaging of Photoluminescent Probes.

Authors: Wan A W Razali (pictured top left), Varun K A Sreenivasan, Carlo Bradac, Mark Connor, Ewa M Goldys and Andrei V Zvyagin.

Abstract: Fluorescence microscopy is a fundamental technique for the life sciences, where biocompatible and photostable photoluminescence probes in combination with fast and sensitive imaging systems are continually transforming this field. A wide-field time-gated photoluminescence microscopy system customised for ultrasensitive imaging of unique nanoruby probes with long photoluminescence lifetime is described. The detection sensitivity derived from the long photoluminescence lifetime of the nanoruby makes it possible to discriminate signals from un-wanted autofluorescence background and laser backscatter by employing a time-gated image acquisition mode. This mode enabled several-fold improvement of the photoluminescence imaging contrast of discrete nanoru-
bies dispersed on a coverslip. It enabled recovery of the photoluminescence signal emanating from discrete na-norubies when covered by a layer of an organic fluorescent dye, which were otherwise invisible without the use of spectral filtering approaches. Time-gated imaging also facilitated high sensitivity detection of nanorubies in a biological environment of cultured cells. Finally, we monitor the binding kinetics of nanorubies to a functionalised substrate, which exemplified a real-time assay in biological fluids. 3D-pseudo colour images of nanorubies immersed in a highly fluorescent dye solution. Nanoruby photolumines-cence is subdued by that of the dye in continuous  excitation/imaging (left), however it can be recovered by time-gated imaging (right). At the bottom is schematic diagram of nanoruby assay in a biological fluid.

The paper is available online.


Minnesota students visit MQ node

Ewa Goldys Low Res Edit 015920 May 2016:

On Friday May 20th 2016, a group of 25 engineering and physics students from the University of Minnesota visited Macquarie University for a very full day of talks, seminars and laboratory tours.

As a part of this visit, CNBP Deputy Director Prof Ewa Goldys provided a 45 minute talk to the students, providing an overview of the Centre and its key research activities.

The future use of nanoparticles, to aid in healthcare and diagnostic capability provoked widespread interest and discussion in the group as they saw the potential benefits of the still evolving technology.

Below: Ewa Goldys explaining the use of nano-rubies and nano-crystals in CNBP related research.



Combining computer analysis with microscopy


CNBP researchers have successfully combined computer analysis with microscopy, to extract highly detailed cellular information that will help distinguish between healthy and diseased cells, in areas as diverse as cancer, injury and inflammation.

The approach, reported in the journal ‘Scientific Reports’, has shown that subtle biochemical signatures of cells can be captured and then categorized, to an extent that has never been seen before.

Paper Title: Quantitative non-invasive cell characterisation and discrimination based on multispectral autofluorescence features.

Authors: Martin E. Gosnell, Ayad G. Anwer, Saabah B. Mahbub, Sandeep Menon Perinchery, David W. Inglis, Partho P. Adhikary, Jalal A. Jazayeri, Michael A. Cahill, Sonia Saad, Carol A. Pollock, Melanie L. Sutton-McDowall, Jeremy G. Thompson & Ewa M. Goldys.

Abstract: Automated and unbiased methods of non-invasive cell monitoring able to deal with complex biological heterogeneity are fundamentally important for biology and medicine. Label-free cell imaging provides information about endogenous autofluorescent metabolites, enzymes and cofactors in cells. However extracting high content information from autofluorescence imaging has been hitherto impossible. Here, we quantitatively characterise cell populations in different tissue types, live or fixed, by using novel image processing and a simple multispectral upgrade of a wide-field fluorescence microscope. Our optimal discrimination approach enables statistical hypothesis testing and intuitive visualisations where previously undetectable differences become clearly apparent. Label-free classifications are validated by the analysis of Classification Determinant (CD) antigen expression. The versatility of our method is illustrated by detecting genetic mutations in cancer, non-invasive monitoring of CD90 expression, label-free tracking of stem cell differentiation, identifying stem cell subpopulations with varying functional characteristics, tissue diagnostics in diabetes, and assessing the condition of preimplantation embryos.

The research paper is accessible online. A CNBP media release is also available.


Review paper published in Nanoscale

Annemarie Nadort26 February 2016:

Dr Annemarie Nadort and CNBP researchers Jiangbo Zhao and Ewa Goldys  have had a review paper accepted for the high-impact journal Nanoscale.

Title: Lanthanide upconversion luminescence at a nanoscale: fundamentals and optical properties

Authors: Annemarie Nadort, Jiangbo Zhao and  Ewa M. Goldys.

Abstract: Upconversion photoluminescence is a nonlinear effect where multiple lower energy excitation photons produce higher energy emission photons. This fundamentally interesting process has many applications in biomedical imaging, light source and display technology, and solar energy harvesting. In this review we discuss the underlying physical principles and their modelling using rate equations. We discuss how the understanding of photophysical processes enabled strategic influence over the optical properties of upconversion especially in rationally designed materials. We subsequently present an overview of recent experimental strategies to control and optimize the optical properties of upconversion nanoparticles, focussing on their emission spectral properties and brightness.

The paper is available online.

Invited talks at University of Colorado Boulder

Ewa Goldys Low Res Edit 015911 February 2016:

Ewa Goldys, CNBP Deputy Director and Lindsay Parker, CNBP Research Fellow were invited speakers at the University of Colorado Boulder.

Ewa and Lindsay, hosted by Profs Steven Meier and Linda Watkins from the Department of Psychology & Neuroscience, spoke about various activities and projects currently taking place across the CNBP. Also discussed were potential areas of research collaboration.

Coverage: Counting cancer-busting oxygen molecules

Ewa Goldys Low Res Edit 01595 February, 2016:

Researchers from the Centre for Nanoscale BioPhotonics (CNBP), have shown that nanoparticles used in combination with X-rays, are a viable method for killing cancer cells deep within the living body.

The research, published in the journal ‘Scientific Reports‘ is based on the successful quantification of singlet oxygen produced during photodynamic therapy for cancer. Singlet oxygen molecules (a highly reactive form of oxygen) are able to kill or inhibit growth of cancer cells in the body due to their toxicity.

Co-lead author on the paper, CNBP Deputy Director Ewa Goldys, provided comment on the work and was featured in a number of media publications including PHYS.ORGGIZMAG and R&D Magazine (all of which are accessible online).