Tag Archives: Dayong Jin

John Booker Medal goes to Dayong Jin

Dayong Jin  Low Res Edit 009617 November 2016:

Congratulations to CNBP Investigator Professor Dayong Jin who has been awarded the 2017 John Booker Medal by the Australian Academy of Science.

Professor Jin was described by the Academy as a world leader in his field, with his research opening up many opportunities in biomedical devices, early diagnosis and light-triggered nanomedicine.

You can read the full story of Professor Jin’s success in an article on the UTS news site.

Paper published in Analytical Chemistry

Xianlin Zheng_web125 February 2016:

CNBP researchers have published a paper in the journal Analytical Chemistry titled, “High-contrast visualization of upconversion luminescence in mice using timegating approach.”

Authors: Xianlin Zheng, Xingjun Zhu, Yiqing Lu, Jiangbo Zhao, Wei Feng, Guohua Jia, Fan Wang, Fuyou Li and Dayong Jin.

Abstract: Optical imaging through the near-infrared (NIR) window provides deep penetration of light up to several centimetres into biological tissues. Capable of emitting 800-nm luminescence under 980-nm illumination, the recently-developed upconversion nanoparticles (UCNPs) suggest a promising optical contrast agent for in vivo bioimaging. However, presently they require high-power lasers to excite when applied to small animals, leading to significant scattering background that limits the detection sensitivity as well as detrimental thermal effect. In this work, we show that the time-gating approach implementing pulsed illumination from a NIR diode laser and time-delayed imaging synchronized via an optical chopper offers detection sensitivity more than one order of magnitude higher than the conventional approach using optical band-pass filters (S/N: 47321/6353 vs. 5339/58), when imaging UNCPs injected into Kunming mice. The pulsed laser illumination (70μs ON in 200 μs period) also reduces the overall thermal accumulation to 35% of that under the continuous-wave mode. Technical details are given on setting up the time-gating unit comprising an optical chopper, a pinhole and a microscopy eyepiece. Being generally compatible with any cameras, this provides a convenient and low cost solution to NIR animal imaging using UCNPs as well as other luminescent probes.

The full paper is accessible online.


Ground breaking hybrid nanocrystal work

Dayong Jin  Low Res Edit 00968 January 2016:

The top ranking journal ‘Nature Communications’ has published the latest ground breaking research from CNBP scientists with a paper titled, ‘Three-dimensional controlled growth of monodisperse sub-50 nm heterogeneous nanocrystals’.

The paper details the creation of  a library of over 800 newly shaped nanocrystals formed from ordered atom clusters over a period of three years. The different shaped or ‘hybrid’ nanocrystals will act as new tools impacting the areas of bio-imaging, diagnostics and nano-medicine.

According to CNBP Chief Investigator, Prof Dayong Jin, the work could form new solutions to getting around the body’s immune system response in the targeted treatment of cancerous cells, which causes both the healthy and diseased cells to die.

The full text of the paper is available online from the Nature Communicatons web site.


Controlling upconversion nanocrystals for emerging applications

Dayong Jin  Low Res Edit 00966 November 2015:

The latest review paper from CNBP researchers Dayong Jin and Bingyang Shi, bench-mark their world-leading roles in upconversion nanotechnoloy.

The paper is titled, “Controlling upconversion nanocrystals for emerging applications.”

Authors: Bo Zhou, Bingyang Shi, Dayong Jin & Xiaogang Liu.

The full paper is available online with the abstract included below.

Abstract: Lanthanide-doped upconversion nanocrystals enable anti-Stokes emission with pump intensities several orders of magnitude lower than required by conventional nonlinear optical techniques. Their exceptional properties, namely large anti-Stokes shifts, sharp emission spectra and long excited-state lifetimes, have led to a diversity of applications. Here, we review upconversion nanocrystals from the perspective of fundamental concepts and examine the technical challenges in relation to emission colour tuning and luminescence enhancement. In particular, we highlight the advances in functionalization strategies that enable the broad utility of upconversion nanocrystals for multimodal imaging, cancer therapy, volumetric displays and photonics.

Bio-conjugation of protein molecules to upconversion nanoparticles

Dayong Jin  Low Res Edit 00962 October 2015:

A novel, simple, and stable method for one-step bio-conjugation of protein molecules to upconversion nanoparticles is demonstrated in the latest paper released by CNBP researchers in the journal ‘Analytical Chemistry’.

The paper is titled “One-step Protein Conjugation to Upconversion Nanoparticles.”

Authors: Jie Lu, Yinghui Chen, Deming Liu, Wei Ren, Yiqing Lu, Yu Shi, James A. Piper, Ian T. Paulsen, and Dayong Jin.

The full paper is available online with the abstract included below.

Abstract: The emerging upconversion nanoparticles offer a fascinating library of ultrasensitive luminescent probes for a range of biotechnology applications from biomarker discovery, single molecule tracking, early disease diagnosis, deep tissue imaging, to drug delivery and therapies.

The effective bio-conjugation of inorganic nanoparticles to the molecule-specific proteins, free of agglomeration, non-specific binding or biomolecule de-activation, is crucial for molecular recognition of target molecules or cells.

The current available protocols require multiple steps which can lead to low probe stability, specificity and reproducibility. Here we report a simple and rapid protein bio-conjugation method based on a one-step ligand exchange using the DNAs as the linker. Our method benefits from the robust DNA-Protein conjugates as well as from multiple ions binding capability.

Protein can be pre-conjugated via an amino group at the 3’ end of a synthetic DNA molecule to the protein, so that the 5′ end phosphoric acid group and multiple phosphate oxygen atoms in the phosphodiester bonds are exposed to replace the oleic acid ligands on the surface of upconversion nanoparticles due to their stronger ions-chelating capability to lanthanides.

We demonstrated our method can efficiently pull out the upconversion nanoparticles from organic solvent into an aqueous phase. The upconversion nanoparticles then become hydrophilic, stable and specific biomolecules recognition. This allows us to successfully functionalize the upconversion nanoparticles with horseradish peroxidise (HRP) for catalytic colorimetric assay and for streptavidin (SA) biotin affinity assays.


In-vivo imaging of Vitamin C

Dayong Jin  Low Res Edit 009618 September 2015:

A new paper has been released in Scientific Reports with two CNBP researchers as contributing authors –  Dayong Jin and Xianlin Zheng. The paper detailed the successfull development of a responsive luminescence probe, TOB-Eu3+, for specific recognition and background-free quantification of vitamin C in living cells and lab animals.

Authors: Bo Song, Zhiqing Ye, Yajie Yang, Hua Ma, Xianlin Zheng, Dayong Jin & Jingli Yuan

Abstract: Sensitive optical imaging of active biomolecules in the living organism requires both a molecular probe specifically responsive to the target and a high-contrast approach to remove the background interference from autofluorescence and light scatterings. Here, a responsive probe for ascorbic acid (vitamin C) has been developed by conjugating two nitroxide radicals with a long-lived luminescent europium complex. The nitroxide radical withholds the probe on its “off” state (barely luminescent), until the presence of vitamin C will switch on the probe by forming its hydroxylamine derivative. The probe showed a linear response to vitamin C concentration with a detection limit of 9.1 nM, two orders of magnitude lower than that achieved using electrochemical methods. Time-gated luminescence microscopy (TGLM) method has further enabled real-time, specific and background-free monitoring of cellular uptake or endogenous production of vitamin C, and mapping of vitamin C in living Daphnia magna. This work suggests a rational design of lanthanide complexes for background-free small animal imaging of biologically functional molecules.

The full paper is accessible online.

Centre CIs win Eureka prize

Eureka27 August 2015:

A team headed up by CNBP Chief Investigators Prof. Dayong Jin and Prof. Tanya Monro has been recognised for its innovative research in developing super-bright nanocrystals (termed Super Dots), winning ‎the Eureka‬ 2015 Prize for Excellence in Interdisciplinary Scientific Research.

The Super Dots team, consisting of Dayong Jin, CNBP, University of Technology Sydney and Macquarie University; Tanya Monro, CNBP, University of South Australia and University of Adelaide; and Bradley Walsh, Minomic International and Macquarie University, was awarded the prize for its work in the creation and use of nanocrystals that can illuminate hidden diseased cells in a living body and which also has application in the labeling, coding and authentication space.

Based on advances in diverse fields including material chemistry, optical physics, nanotechnology, biotechnology, computational modelling and instrumentation engineering, Super Dot technology is truly interdisciplinary in nature explained Prof. Jin.

“Our invention is a typical example of an interdisciplinary approach.  By sourcing advances in physics, chemistry and biology, we have been able to breakthrough bottleneck issues in material sciences and optical physics, allowing us to develop a new transformational technology.”

He added, “Research within a single discipline is interesting, but interdisciplinary research, working with collaborators with a shared focus as a team, is far more exciting and rewarding.”

Additional information on this winning team is available online from the Australian Museum – Eureka web site.




Multifunctional lanthanide nanomaterials paper

Dayong Jin  Low Res Edit 009621 August 2015:

CNBP researchers Prof. Dayong Jin and Dr. Yong Liu feature on a recently released publication from Scientific Reports published by the Nature Publication Group.

The paper titled “Multifunctional luminescent nanomaterials from NaLa(MoO4)2:Eu3+/Tb3+ with tunable decay lifetimes, emission colors, and enhanced cell viability”, can be downloaded in its entirety.

Abstract: A facile, but effective, method has been developed for large-scale preparation of NaLa(MoO4)2 nanorods and microflowers co-doped with Eu3+ and Tb3+ ions (abbreviated as: NLM:Ln3+). The as-synthesized nanomaterials possess a pure tetragonal phase with variable morphologies from shuttle-like nanorods to microflowers by controlling the reaction temperature and the amount of ethylene glycol used. Consequently, the resulting nanomaterials exhibit superb luminescent emissions over the visible region from red through yellow to green by simply changing the relative doping ratios of Eu3+ to Tb3+ ions. Biocompatibility study indicates that the addition of NLM:Ln3+ nanomaterials can stimulate the growth of normal human retinal pigment epithelium (ARPE-19) cells. Therefore, the newly-developed NaLa(MoO4)2 nanomaterials hold potentials for a wide range of multifunctional applications, including bioimaging, security protection, optical display, optoelectronics for information storage, and cell stimulation.

Authors: Mei Yang, Youlong Liang, Qingyuan Gui, Bingxin Zhao, Dayong Jin, Mimi Lin, Lu Yan, Hongpeng You, Liming Dai, and Yong Liu.


Novel scaffolds for MC3T3-E1 cell stimulation and drug release

Dayong Jin  Low Res Edit 009610 August 2015:

CNBP researchers Prof. Dayong Jin and Dr. Yong Liu feature on a newly published paper titled, “Multifunctional chitosan/polyvinyl pyrrolidone/45S5 Bioglass® scaffolds for MC3T3-E1 cell stimulation and drug release.” The paper was released in the journal, ‘Materials Science and Engineering C: Materials for Biological Applications.’

Abstract: Novel chitosan–polyvinyl pyrrolidone/45S5 Bioglass® (CS-PVP/BG) scaffolds were prepared via foam replication and chemical cross-linking techniques. The pristine BG, CS-PVP coated BG and genipin cross-linked CS-PVP/BG (G-CS-PVP/BG) scaffolds were synthesized and characterized in terms of chemical composition, physical structure and morphology respectively. Resistance to enzymatic degradation of the scaffold is improved significantly with the use of genipin cross-linked CS-PVP. The bio-effects of scaffolds on MC3T3-E1 osteoblast-like cells were evaluated by studying cell viability, adhesion and proliferation. The CCK-8 assay shows that cell viability on the resulting G-CS-PVP/BG scaffold is improved obviously after cross-linking of genipin. Cell skeleton images exhibit that well-stretched F-actin bundles are obtained on the G-CS-PVP/BG scaffold. SEM results present significant improvement on the cell adhesion and proliferation for cells cultured on the G-CS-PVP/BG scaffold. The drug release performance on the as-synthesized scaffold was studied in a phosphate buffered saline (PBS) solution. Vancomycin is found to be released in burst fashion within 24 h from the pristine BG scaffold, however, the release period from the G-CS-PVP/BG scaffold is enhanced to 7 days, indicating improved drug release properties of the G-CS-PVP/BG scaffold. Our results suggest that the G-CS-PVP/BG scaffolds possess promising physicochemical properties, sustained drug release capability and good biocompatibility for MC3T3-E1 cells’ proliferation and adhesion, suggesting their potential applications in areas such as MC3T3-E1 cell stimulation and bone tissue engineering.

Qingqing Yao, Wei Li, Shanshan Yu, Liwei Ma, Dayong Jin, Aldo R. Boccaccini, Yong Liu, Materials Science and Engineering: C, 2015, 56, 473-480.

The full article can be downloaded here: http://www.sciencedirect.com/science/article/pii/S092849311530179X

CNBP makes mark at APNFO10

Wan Razali16 July 2015:

The 10th Asia-Pacific Conference on Near-field Optics (APNFO10), held July 7-10 2015 in Japan, saw significant CNBP representation and visibility.

Two Centre Chief Investigators gave invited talks:

Dayong Jin (University of Technology, Sydney) – Upconversion SuperDots for Nanoscale Biophotonics. 

Brant Gibson (RMIT University) – Nanodiamonds: Hybrid-Photonic and BioPhotonic applications.

CNBP PhD student Wan Aizuddin Wan Razali (pictured) also won the IAC Award at  APNFO10 for top Student Oral with Poster presentation. His topic – “Ultrasensitive Nanoruby Imaging using Time gated Luminescence Microscopy.”

Full conference details can be found here.