Tag Archives: David Inglis

New probe to detect hydrogen peroxide

10 June 2019:

A team of CNBP researchers have published a new paper discussing the design and application of a micro fabricated needle-like probe to measure hydrogen peroxide.  This new microfluidic tool has applications for monitoring dynamic chemical reactions in analytical chemistry and biological systems.

Journal: RSC Advances

Publication Title: Microfabricated needle for hydrogen peroxide detection

Authors: Shilun Feng, Sandhya Clement, Yonggang Zhu, Ewa M. Goldys and David W. Inglis

Abstract:  A microfabricated needle-like probe has been designed and applied for hydrogen peroxide (H2O2) sampling and detection using a commercial, single-step fluorescent H2O2 assay. In this work, droplets of the assay reagent are generated and sent to the needle tip using a mineral-oil carrier fluid. At the needle tip, the sample is drawn into the device through 100 mm long hydrophilic capillaries by negative pressure. The sampled fluid is immediately merged with the assay droplet and carried away to mix and react, producing a sequence of droplets representing the H2O2 concentration as a function of time. We have characterized the assay fluorescence for small variations in the sample volume. With the calibration, we can calculate the concentration of H2O2 in the sampled liquid from the size and intensity of each merged droplet. This is a microfluidic data-logger system for on-site continuous sampling, controlled reaction, signal storage and on-line quantitative detection. It is a useful tool for monitoring dynamic chemical reactions in analytical chemistry and biological applications.

Key words: Microfluidics, probe, H2O2, analytics chemistry

Microfluidic droplet extraction

16 November 2017:

CNBP and Macquarie University PhD candidate Shilun Feng is first author on a new paper exploring a ‘membrane-on-a-chip’ device. The technology has the potential to form an integral part of a new type of microneedle that would be able to transport tiny and precise amounts of fluid/medication within the body.

Journal: Micromachines.

Publication titleMicrofluidic Droplet Extraction by Hydrophilic Membrane.

Authors: Shilun Feng, Micheal N. Nguyen, and David W. Inglis.

Abstract: Droplet-based microfluidics are capable of transporting very small amounts of fluid over long distances. This characteristic may be applied to conventional fluid delivery using needles if droplets can be reliably expelled from a microfluidic channel. In this paper, we demonstrate a system for the extraction of water droplets from an oil-phase in a polymer microfluidic device. A hydrophilic membrane with a strong preference for water over oil is integrated into a droplet microfluidic system and observed to allow the passage of the transported aqueous phase droplets while blocking the continuous phase. The oil breakthrough pressure of the membrane was observed to be 250 ± 20 kPa, a much greater pressure than anywhere within the microfluidic channel, thereby eliminating the possibility that oil will leak from the microchannel, a critical parameter if droplet transport is to be used in needle-based drug delivery.

Maximizing particle concentration

28 April 2017:

A new paper from CNBP researchers reports on an improvement to deterministic lateral displacement arrays, which allows for higher particle concentration enhancement. The work has just been published in the journal ‘Biomicrofluidics’ and is accessible online.

Journal: Biomicrofluidics.

Title: Maximizing particle concentration in deterministic lateral displacement arrays.

Authors: Shilun Feng, Alison M. Skelley, Ayad G. Anwer (pictured top left), Guozhen Liu and David W. Inglis.

Abstract: We present an improvement to deterministic lateral displacement arrays, which allows higher particle concentration enhancement. We correct and extend previous equations to a mirror-symmetric boundary. This approach allows particles to be concentrated into a central channel, no wider than the surrounding gaps, thereby maximizing the particle enrichment. The resulting flow patterns were, for the first time, experimentally measured. The performance of the device with hard micro-spheres and cells was investigated. The observed flow patterns show important differences from our model and from an ideal pattern. The 18 μm gap device showed 11-fold enrichment of 7 μm particles and nearly perfect enrichment—of more than 50-fold—for 10 μm particles and Jurkat cells. This work shows a clear path to achieve higher-than-ever particle concentration enhancement in a deterministic microfluidic separation system.