A tuneable and programmable integrating sphere light source for wide-field fluorescent microscopy imaging, employing nine light-emitting diodes (LEDs), has been successfully demonstrated and reported by CNBP researchers in a new paper (lead author Aziz ul Rehman pictured).
The paper, published in ‘Photodiagnosis and Photodynamic Therapy’ is accessible online.
Journal: Photodiagnosis and Photodynamic Therapy.
Publication title: Programmable LED-Based Integrating Sphere Light Source for Wide-Field Fluorescence Microscopy.
Authors: Aziz ul Rehman, Ayad G.Anwer, Ewa M.Goldys.
Abstract: Wide-field fluorescence microscopy commonly uses a mercury lamp, which has limited spectral capabilities. We designed and built a programmable integrating sphere light (PISL) source which consists of nine LEDs, light-collecting optics, a commercially available integrating sphere and a baffle. The PISL source is tuneable in the range 365–490 nm with a uniform spatial profile and a sufficient power at the objective to carry out spectral imaging. We retrofitted a standard fluorescence inverted microscope DM IRB (Leica) with a PISL source by mounting it together with a highly sensitive low- noise CMOS camera. The capabilities of the setup have been demonstrated by carrying out multispectral autofluorescence imaging of live BV2 cells.