Tag Archives: andrew care

CNBP researchers edit new book

30 October 2017:

A new book edited by A/Prof Anwar Sunna (CNBP Associate Investigator), Dr Andrew Care (CNBP Research Fellow) and Peter Bergquist (Macquarie University) as been published by Springer.

The book, “Peptides and Peptide-based Biomaterials and their Biomedical Applications”, highlights new developments in the applications of peptide and peptide-based biomaterials in biomedicine.

“This is a fast-moving and rapidly expanding research area, which promises to be one of the most significant fields of research in applied biomedicine”, says A/Prof Sunna.

“The work introduces readers to direct applications and translational research at the interface between materials science, protein chemistry and biomedicine.”

Immobilization of enzymes onto solid supports

4 August 2017:

The study on “Solid-binding peptides for immobilization of thermostable enzymes to hydrolyze biomass polysaccharides” by CNBP Researcher Dr Andrew  Care and led by CNBP Associate Investigator  A/Prof Anwar Sunna has been featured on Renewable Energy Global Innovations as a key scientific paper.

The work was originally published in the scientific journal Biotechnology for Biofuels (February, 2017).

 

CNBP talks to the pollies at SmP

24 March 2017:

A chance to talk science with Australian politicians and policy influencers was an opportunity seized by CNBP with Centre Investigator Prof Heike Ebendorff-Heidepriem and Centre Research Fellow Dr Andrew Care both in attendance at the annual ‘Science meets Parliament’ (SmP) event, Canberra, 21-22 March, 2017.

Established by Science and Technology Australia, SmP provides 200 scientists with a unique professional development opportunity to get a clear sense of the competing rationalities of science, politics and public policy. The two-day gathering also includes a day at Parliament House, where delegates get the chance to meet privately with parliamentarians.

As part of this activity, Prof Ebendorff-Heidepriem met with Senator Chris Back and Senator Chris Ketter, and also spoke with Shadow Minister of Defence, Richard Marles. In addition, she spoke with many researchers and entrepreneurs from both the University and industry sectors.

“Improving collaboration between the research community and industry was a hot topic in many of the discussions that I had”, said Heike. “Particularly in my meeting with Senator Chris Back. People were also extremely excited about our approach, in using fibres and light to create exciting new windows into the body.”

CNBP’s Dr Andrew Care met with Opposition Leader Bill Shorten’s advisor, discussing gender equality and early education for STEM and also touching on ECR opportunities and improving research and industry ties. He also met MP Adam Bandt, the Greens spokesperson for science.

“Overall it was an extremely rewarding experience,” says Andrew. “Attending SmP gave me the opportunity to explore the political process and to network with many other researchers from academia, industry, and governance. It was fantastic to see science and innovation so high on the government’s agenda.”

A full round up from both days of SmP can be found on the STA web site – Day 1 and Day 2.

Below – MP Adam Bandt and CNBP’s Dr Andrew Care.

 

Deep-penetrating photodynamic therapy

4 January 2017:

CNBP researchers (Liuen Liang pictured), report on the deployment of upconversion nanoparticles to enhance the treatment depth of the fluorescent protein KillerRed in photodynamic therapy.

The work was published in the journal ‘Acta Biomaterialia’ and is accessible online.

Journal: Acta Biomaterialia.

Title: Deep-penetrating photodynamic therapy with KillerRed mediated by upconversion nanoparticles.

Authors: Liuen Liang, Yiqing Lu, Run Zhang, Andrew Care, Tiago A. Orteg, Sergey M. Deyev, Yi Qian, Andrei V. Zvyagina.

Abstract: The fluorescent protein KillerRed, a new type of biological photosensitizer, is considered as a promising substitute for current synthetic photosensitizes used in photodynamic therapy (PDT). However, broad application of this photosensitiser in treating deep-seated lesions is challenging due to the limited tissue penetration of the excitation light with the wavelength falling in the visible spectral range. To overcome this challenge, we employ upconversion nanoparticles (UCNPs) that are able to convert deep-penetrating near infrared (NIR) light to green light to excite KillerRed locally, followed by the generation of reactive oxygen species (ROS) to kill tumour cells under centimetre-thick tissue. The photosensitizing bio-nanohybrids, KillerRed-UCNPs, are fabricated through covalent conjugation of KillerRed and UCNPs. The resulting KillerRed-UCNPs exhibit excellent colloidal stability in biological buffers and low cytotoxicity in the dark. Cross-comparison between the conventional KillerRed and UCNP-mediated KillerRed PDT demonstrated superiority of KillerRed-UCNPs photosensitizing by NIR irradiation, manifested by the fact that ∼70% PDT efficacy was achieved at 1-cm tissue depth, whereas that of the conventional KillerRed dropped to ∼7%.

Rapidly labeling antibodies with luminescence

Labelled cells10 June 2016:

CNBP and Macquarie University researchers have successfully developed a novel indirect universal detection reagent that rapidly labels antibodies with luminescence within seconds and without the need for any complicated bioconjugation procedures. The reagent, reported in the journal ‘Scientific Reports’, can be used to directly label antibodies for several time-gated luminescence applications, e.g., bioimaging, cell labeling and detection, and flow cytometry.

The work was co-authored by CNBP researchers Dr Andrew Care and Dr Nima Sayyadi and led by CNBP Associate Investigator Dr Anwar Sunna.

Pictured (top left) – giardia cyst cells labeled with europium chelate.

Publication title: A Novel Universal Detection Agent for Time-Gated Luminescence Bioimaging

Authors: Nima Sayyadi and Andrew Care ( first co-authors), Russell E. Connally, Andrew C. Try, Peter L. Bergquist and Anwar Sunna

Abstract:
Luminescent lanthanide chelates have been used to label antibodies in time-gated luminescence (TGL) bioimaging. However, it is a challenging task to label directly an antibody with lanthanide-binding ligands and achieve control of the target ligand/protein ratios whilst ensuring that affinity and avidity of the antibody remain uncompromised. We report the development of a new indirect detection reagent to label antibodies with detectable luminescence that circumvents this problem by labelling available lysine residues in the linker portion of the recombinant fusion protein Linker-Protein G (LPG). Succinimide-activated lanthanide chelating ligands were attached to lysine residues in LPG and Protein G (without Linker) and the resulting Luminescence-Activating (LA-) conjugates were compared for total incorporation and conjugation efficiency. A higher and more efficient incorporation of ligands at three different molar ratios was observed for LPG and this effect was attributed to the presence of eight readily available lysine residues in the linker region of LPG. These Luminescence-Activating (LA-) complexes were subsequently shown to impart luminescence (upon formation of europium(III) complexes) to cell-specific antibodies within seconds and without the need for any complicated bioconjugation procedures. The potential of this technology was demonstrated by direct labelling of Giardia cysts and Cryptosporidium oocysts in TGL bioimaging.

The paper is available online.

CNBP at US-Australia Technical Exchange meeting

Heike Ebendorff Low Res Edit 018023 May 2016:

Professor Heike Ebendorff-Heidepriem (CNBP Investigator), Dr Lindsay Parker (CNBP Research Fellow) and Dr Andrew Care (CNBP Research Fellow) spoke at the US-Australia Enabling Technologies Technical Exchange Meeting 2016 at UNSW in Sydney on 23-24 May 2016.

Heike’s talk was: “Pushing the Limits in Glass Properties and Structures for Laser, Sensing and Nonlinearity Applications.”

Lindsay’s talk was: “Illuminating mRNA and proteins in new ways with nanoparticles and chemical conjugates.”

Andrew’s talk was: “Developing a platform technology for the self-assembly of functional nanoparticles.”

The purpose of the Technical Exchange is to explore and potentially develop new areas of basic research collaboration between Australian and US participants.

Additional meeting information is available online.

 

Chapter on solid-binding peptides to be published

Andrew Care Low Res Edit 017214 July 2015:

CNBP researchers feature in the publication of an exciting new book chapter, highlighting the use of solid-binding peptides in the immobilisation of enzymes onto solid materials for applications in biotechnology.

To be published  in the Springer book series “Grand Challenges in Biology and Biotechnology”, the chapter is titled ‘Solid-Binding Peptides: New Immobilisation Strategies for Extremophile Biocatalysis in Biotechnology’.

Authors: Andrew Care, Peter L. Bergquist and Anwar Sunna

 

Invited Talk at International Nanomedicine Conference

Andrew Care Low Res Edit 01726-8 July 2015:

Dr Andrew Care has been invited to give a talk at the 6th International Nanomedicine Conference, to be held in Sydney July 6 -8 2015.

He will give an oral presentation titled: ‘Building a platform technology for the self-assembly of functional upconversion nanoparticles.’A. Care, A. Sunna and N. Packer

Organised by the Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology.

Additional conference information available here – http://www.oznanomed.org/