Tag Archives: Abdulrahman Shathili

Understanding glycome changes in diabetic ovarian tissue

28 August 2018:

This paper describes the characterization of protein glycosylation in the ovary and measures the changes that occur with the induction of diabetes. The lead author on the paper is CNBP PhD student Abdulrahman M Shathili from Macquarie University (pictured).

Journal: Glycobiology.

Publication title: The effect of streptozotocin-induced hyperglycemia on N-and O-linked protein glycosylation in mouse ovary.

Authors: Abdulrahman M Shathili, Hannah M Brown, Arun V Everest-Dass, Tiffany C Y Tan, Lindsay M Parker, Jeremy G Thompson, Nicolle H Packer.

Abstract: Post-translational modification of proteins namely glycosylation influences cellular behavior, structural properties and interactions including during ovarian follicle development and atresia. However, little is known about protein glycosylation changes occurring in diabetes mellitus in ovarian tissues despite the well-known influence of diabetes on the outcome of successful embryo implantation. In our study, the use of PGC chromatography–ESI mass spectrometry in negative ion mode enabled the identification of 138 N-glycans and 6 O-glycans on the proteins of Streptozotocin-induced (STZ) diabetic mouse ovarian tissues (n = 3). Diabetic mouse ovaries exhibited a relative decrease in sialylation, fucosylation and, to a lesser extent, branched N-linked glycan structures, as well as an increase in oligomannose structures on their proteins, compared with nondiabetic mouse ovaries. Changes in N-glycans occurred in the diabetic liver tissue but were more evident in diabetic ovarian tissue of the same mouse, suggesting an organ-specific effect of diabetes mellitus on protein glycosylation. Although at a very low amount, O-GalNAc glycans of mice ovaries were present as core type 1 and core type 2 glycans; with a relative increase in the NeuGc:NeuAc ratio as the most significant difference between control and diabetic ovarian tissues. STZ-treated mice also showed a trend towards an increase in TNF-α and IL1-B inflammatory cytokines, which have previously been shown to influence protein glycosylation.

CNBP researchers at National Proteomics Conference

Nicki Packer Low Res Edit 01257 February 2016:

The 21st Annual Lorne Proteomics Symposium, organized by the Australasian Proteomics Society, was held February 4th – 7th 2016 in Lorne, Victoria and was attended by CNBP members of the Macquarie Node, Nicolle Packer, Christopher Ashwood and Abdulrahman Shathili.

The symposium featured the latest developments in proteomics technologies and tools for the interpretation of proteomics outputs, and their application toward answering fundamental questions in biology. Invited speakers included the most cited person in the field of proteomics, Matthias Mann, as well as internationally renowned glycomics expert, Anne Dell. CNBP Chief Investigator Nicolle Packer was also an invited speaker at the event and presented a talk titled “Alterations in Glycosylation in Ovarian Cancer.”

Students Christopher Ashwood and Shathili Abdulrahman both presented posters describing results from the past year, with Christopher Ashwood being awarded a poster prize for his research on characterisation of novel protein-glycosylation using rarely used analytical techniques in the field of glycomics.

Symposium information can be found online.