How do you measure the tenderness, juiciness and flavour of beef without eating it? It’s a challenge faced by everyone from meat inspectors at abattoirs to consumers looking through a butcher’s window. And they’re all subjective – until now. Continue reading
The week culminated in gala where researchers were recognised for their achievements through awards ranging from academic excellence to commercial impact, outreach and collaboration.
Read about the awardees below, and congratulations to all our members who were recognised for their achievements in 2019!
2019 Academic Excellence Award Transdisciplinary Research Publication
Awarded to the best 2019 publication connecting CNBP researchers from multiple disciplines. This year’s award recognises a collaboration between researchers working with nanoparticles, microscopy, computational imaging and molecular & cellular biology.
Denkova, D., M. Ploschner, M. Das, L. M. Parker, X. Zheng, Y. Lu, A. Orth, N. H. Packer and J. A. Piper (2019). “3D sub-diffraction imaging in a conventional confocal configuration by exploiting super-linear emitters.” Nat Commun 10(1): 3695.
2019 Academic Excellence Award – International Impact
Awarded to the best 2019 publication connecting CNBPs Australian researchers with International Partners. This year’s award recognises collaboration between researchers at RMIT University and QST, Japan.
Capelli, M., A. H. Heffernan, T. Ohshima, H. Abe, J. Jeske, A. Hope, A. D. Greentree, P. Reineck and B. C. Gibson (2019). “Increased nitrogen-vacancy centre creation yield in diamond through electron beam irradiation at high temperature.” Carbon 143: 714-719.
2019 Academic Excellence Award – Best Student Publication
Awarded to the best 2019 publication first authored by a CNBP student as determined by journal impact factor.
Wei, Y., H. Ebendorff‐Heidepriem and J. Zhao (2019). “Recent Advances in Hybrid Optical Materials: Integrating Nanoparticles within a Glass Matrix.” Advanced Optical Materials. DOI: 10.1002/adom.201900702
2019 Academic Excellence Award – Best Researcher (non-student) Publication
Awarded to the best 2019 publication as determined by journal impact factor.
Jia, P., K. Zuber, Q. Guo, B. C. Gibson, J. Yang and H. Ebendorff-Heidepriem (2019). “Large-area freestanding gold nanomembranes with nanoholes.” Materials Horizons 6(5): 1005-1012. DIO:10.1039/c8mh01302k
2019 Highest Altmetrics – Social Impact
Awarded to the 2019 publication recognised by the wider non-academic community, as determined by Altmetric score.
Habibalahi, A., C. Bala, A. Allende, A. G. Anwer and E. M. Goldys (2019). “Novel automated non invasive detection of ocular surface squamous neoplasia using multispectral autofluorescence imaging.” Ocular Surface. DOI:10.1016/j.jtos.2019.03.003
2019 Quality Communication Award – Social Media
2019 Quality Communication Award – Engagement in Centre Outreach Activity
Awarded to Dr Lindsay Parker for community engagement activities including: Science in the Swamp, Exploring Brain Research at Castle Hill Library; and multiple engagements with school groups in Sydney and her home town in the USA.
2019 Nurturing Environment Award – Mentor
Awarded to Professor Andrew Greentree for commitment to mentorship and contribution to CNBP professional development activities including the PhD Publication’s Masterclass, Research integrity training and supporting fellowship applications.
2019 Nurturing Environment Award – 5% Commitment to CNBP
This award recognises individuals that go above and beyond CNBPs requirement for researchers to commit 5% of their time to non-research activities. Awarded to Dr Georgina Sylva for ongoing commitment to science outreach in regional and remote communities.
2019 Commercial Impact Award – Individual Engagement with Industry/End-users
Awarded to a CNBP researcher for successful collaboration with industry and end-users. Prof Heike Ebendorff-Heidepriem for her disruptive glass projects with multiple industry partners and her collaborations with the glass-art community.
2019 Commercial Impact Award – CNBP Project
Awarded for the most successful CNBP research project – industry collaboration. Dr Thomas Avery and Associate Professor Peter Grace for next gen non-opioid non addictive pain therapies.
2019 Photo/Video Competition Award
Awarded to the photo captured by Suliman Yagoub: Embryo staining for DNA repair #MicroFireWorks #Embryology @UniofAdelaide @UofA_embros
— Suliman Yagoub (@SulimanYagoub) October 8, 2019
2019 Pitch Fest Award
CNBP pitch fest is the brain child of the ECR led Entrepreneurs Network. After participation in a CNBP-led pitching workshop, Individuals pitch their idea/project to the wider CNBP community with pitches judged by an expert panel.
Equal first prize:
Mr Suliman Yagoub: Towards Automation of in vitro Fertilization (IVF) Treatment.
Current IVF success requires skilled embryologists to perform regular, routine procedures. By automating and standardizing IVF procedures we will reduce human error for IVF treatments world-wide
Dr Andrew Care and A/Prof Lyndsay Collins-Praino: Intercepting Parkinson’s Disease
This novel technology employs bioengineered nanoparticles to halt the progression of Parkinson’s Disease inside the human brain.
2019 Annual Conference – Best Poster Award
Awarded to the best CNBP conference poster by popular vote. Aimee Horsfall – Poster: Enhancing protein biosensor sensitivity requires detailed structural insight.
2019 Director’s Award
Each year the Prof Mark Hutchinson identifies the individual(s) who’s contribution to the centre and/or support to the Director has stood out. Joint award to the CNBP Deputy Directors: Prof Brant Gibson & Prof Ewa Goldys for all-round awesome!
Researchers have found a way to identify multiple cell signalling proteins using a single cell rather than the billions of cells used previously.
The new measurement technology, developed by researchers at the ARC Centre of Excellence for Nanoscale Biophotonics, brings precision medicine a step closer.
“Cells secrete various messenger molecules, such as cytokines. They may indicate the presence of a disease or act as a driver of key therapeutic effects,” says Dr Guozhen Liu, lead author of paper detailing the technology.
The method, termed OnCELISA, uses antibodies attached on specially engineered cell surfaces to capture cytokine molecules before they have a chance to disperse away from the cell.
The secreted messenger proteins such as cytokines are reported, at the single cell level, by using fluorescent magnetic nanoparticles.
Cytokines secreted from cells play a critical role in controlling many physiological functions, including immunity, inflammation, response to cancer, and tissue repair.
The OnCELISA system can be used for ultrasensitive monitoring of cytokine release by individual cells, and it can also help discover cell populations with therapeutic value.
“The ability to identify and select cell populations based on their cytokine release is particularly valuable in commercial cell technologies and it can help develop unique products, such as future non-opioid pain relief” says Dr Liu.
“Importantly, our design uses commercially available reagents only, so it can be easily reproduced by others,” she adds.
While the published work focuses on specific proinflammatory cytokines IL-6 and IL-1β, the method is potentially suitable for a broad range of other secreted proteins and cell types.
The new technique represents an advance on traditional methods such as the enzyme-linked immunosorbent assays (ELISA) that detect average levels of secreted molecules from cell ensembles.
The OnCELISA takes the ELISA approach to its absolute extreme, by detecting cytokines on the surface of individual, single live cells.
The publication has been reported by prestigious iScience journal and can be found at https://www.sciencedirect.com/science/article/pii/S2589004219303578.
Publication Title: A Nanoparticle-Based Affinity Sensor that Identifies and Selects Highly Cytokine-Secreting Cells
Authors: Guozhen Liu; Christina Bursill; Siân P.Cartland; Ayad G.Anwer; Lindsay M.Parker; Kaixin Zhang; Shilun Feng; Meng He; David W.Inglis; Mary M.Kavurma; Mark R.Hutchinson; Ewa M.Goldys
Summary: We developed a universal method termed OnCELISA to detect cytokine secretion from individual cells by applying a capture technology on the cell membrane. OnCELISA uses fluorescent magnetic nanoparticles as assay reporters that enable detection on a single-cell level in microscopy and flow cytometry and fluorimetry in cell ensembles. This system is flexible and can be modified to detect different cytokines from a broad range of cytokine-secreting cells. Using OnCELISA we have been able to select and sort highly cytokine-secreting cells and identify cytokine-secreting expression profiles of different cell populations in vitro and ex vivo. We show that this system can be used for ultrasensitive monitoring of cytokines in the complex biological environment of atherosclerosis that contains multiple cell types. The ability to identify and select cell populations based on their cytokine expression characteristics is valuable in a host of applications that require the monitoring of disease progression.
CNBP has officially welcomed UNSW, one of the world’s leaders at translational engineering research, as its newest node.
In addition to the official open by UNSW Engineering Dean Professor Mark Hoffman, CNPB Director Professor Mark Hutchinson took the opportunity to lay out the CNBP mission and its accomplishments at an industry showcase. Continue reading
The CNBP and its researchers are taking part in a wide range of activities for National Science Week.
This Thursday 8 August researcher Dr Wei Deng from UNSW Sydney will explain how nanotechnogy is changing how we treat cancer, as part of Inspiring Australia’s Talking Science series.
It will be held at the Max Webber Library, in Blacktown, Sydney. More details here.
On Sunday, 11 August, Adelaide University’s Lyndsey Collins-Praino will host Kids Navigate Neuroscience, an event at which children aged 4-10 can explore how the brain works in a fun and hands-on way by participating in a series of interactive neuroscience exhibits.
On Tuesday 13 August explore medical brain research by joining Dr Lindsay Parker, a researcher at Macquarie University, as she discusses how she is trying to create better medicines for Alzheimer’s, chronic pain and brain cancer, by only targeting the unhealthy cells in the brain.
This event is part of Inspiring Australia’s Talking Science series as part of National Science Week. Bookings available now. Contact details:
Castle Hill Library
The Hills Shire Library Service
Phone: 02 9761 4510
There is a fun evening next Friday, 16 August, at the Adelaide Medical School, University of Adelaide, where you can explore the neuroscience of sex, drugs and salsa dancing.
A series of interactive exhibits will address questions such as, what role does the brain play in sexual attraction? Can you salsa dance your way to a healthy brain? How does the brain perceive different flavours when drinking wine, and how can pairing wine with different foods alter this perception?
Also next Friday, 16 August, the whole family is invited to see some amazing short videos on a massive screen in a free National Science Week Event hosted by STEMSEL Foundation Braggs Lecture Theatre, University of Adelaide AI Light Science Spectacular.
You will find out how the eye works, how NASA finds planets in other solar systems and how detected the edge of the Universe.
You will also explore light, from nanoscale biophotonics with CNBP research fellow Dr Roman Kostecki to exploring the Universe with Dr Jerry Madakbas, a photonics physicist who builds night vision sensors for NASA.
You can book through Eventbrite.
Also on Friday night:
What role does the brain play in sexual attraction? Can you salsa dance your way to a healthy brain? How does the brain perceive different flavours when drinking wine, and how can pairing wine with different foods alter this perception?
These days, you can’t seem to walk through the aisle of a grocery store without being bombarded by newspaper and magazine headlines touting the latest and greatest breakthrough in neuroscience research. But how can you tell fact from fiction?
Join us for this Big Science Adelaide event, held at the Adelaide Health and Medical Sciences (AHMS) building at the University of Adelaide, where we’ll explore the answers to these questions and many more!
More details at https://www.scienceweek.net.au/neuroscience-at-night/
Finally, CNBP researchers will be taking part in Science in the Swamp, a fun, free family festival of science displays, shows and activities on Sunday 18 August in Centennial Park, Sydney.
Join scientists as they show what amazing superpowers you find in nature – super sight, super hearing, super strength and camouflage are only some of the capabilities on show.
Be sure to put on your cape and dress up as your favourite superhero for this great event. You can find out more details here.
A team led by the CNBP’s Dr Guozhen Liu has developed intelligent biodegradable polymer nanoparticles, which can help monitor a cell-signalling protein, or cytokine, widely expressed in cancer cells. The technique can help with earlier diagnostics and even treatment and represents another step towards personalised nanomedicine.
The research integrates a specific fluorogen – a molecule that generates fluorescence and can be used for protein monitoring – with PLGA nanoparticles for the first time.
The fluorogen in question is a so-called “aggregation-induced emission” fluorogen, known as an AIEgen. Aggregation-induced emission (AIE), has become an important area of research since its discovery around 20 years ago. It describes an abnormal phenomenon, in which some compounds show greater fluorescence as they aggregate than when in solution, as is more common. These AIEgens provide superior advantages for biosensing and bioimaging.
The integration of the nanoparticle and the AIEgen could become an important tool in the relatively new field of medicine known as “theranostics” – a combination of “therapy” and “diagnostics” made possible through the use of nanoparticles and an important transition towards personalised medicine.
Dr Liu’s discovery, for example, detects high levels of the cytokine VEGF-A found in tumor cells, and monitors simultaneous photothermal therapy (PTT), in which heat is used to kill cancer cells, and magnetic resonance imaging (MRI) as part of a whole package of early diagnostics and treatment of cancer cells.
It could be used in the future as a smart drug delivery system, with cancer drugs loaded in the nanoparticles for controlled and sustained release targeted precisely to a tumor.
In the future, Dr Liu believes it will be possible to develop the next generation of intelligent nanoparticles which can continually monitor cytokines and cytokine-triggered drug delivery while also carrying out deep tissue imaging.
Dr Liu is an ARC Future Fellow and Senior Lecturer at Graduate School of Biomedical Engineering at UNSW.
You can read the paper here.
Publication Title: AIEgen based poly(L-lactic-co-glycolic acid) magnetic nanoparticles to localize cytokine VEGF for early cancer diagnosis and photothermal therapy
Authors: Ma, K (Ma, Ke); Liu, GJ (Liu, Guo-Jun); Yan, LL (Yan, Lulin); Wen, SH (Wen, Shihui); Xu, B (Xu, Bin); Tian, WJ (Tian, Wenjing); Goldys, EM (Goldys, Ewa M.); Liu, GZ (Liu, Guozhen)
Abstract: Aim: We demonstrated a novel theranostic system for simultaneous photothermal therapy and magnetic resonance imaging applicable to early diagnostics and treatment of cancer cells. Materials & methods: Oleic acid-Fe3O4 and triphenylamine-divinylanthracene-dicyano were loaded to the poly(L-lactic-co-glycolic acid) nanoparticles (NPs) on which anti-VEGF antibodies were modified to form anti-VEGF/OA-Fe3O4/triphenylamine-divinylanthracene-dicyano@poly(L-lactic-co-glycolic acid) NPs. The 1H nuclear magnetic resonance (NMR), mass spectra, fluorescence, UV absorption, dynamic light scattering, transmission electron microscope and inductively coupled plasma mass spectrometry tests were used to characterize the NPs, and the bioimaging was illustrated by confocal laser scanning microscope (CLSM) and in vivo MRI animal experiment. Results: This system was capable to recognize the overexpressed VEGF-A as low as 68pg/ml in different cell lines with good selectivity and photothermal therapy effect. Conclusion: These ultrasensitive theranostic NPs were able to identify tumor cells by fluorescence imaging and MRI, and destroy tumors under near infrared illumination.
Author Keywords: AIEgen; cytokines; MRI; PDT; PLGA nanoparticle; PTT; theranostics
KeyWords Plus: ENDOTHELIAL GROWTH-FACTOR; IN-VIVO; ANGIOGENESIS; THERANOSTICS; NANOSPHERES; APTASENSOR; EXPRESSION; PROGNOSIS; MEDICINE; PROBE
A team of CNBP researchers have published a new paper discussing the design and application of a micro fabricated needle-like probe to measure hydrogen peroxide. This new microfluidic tool has applications for monitoring dynamic chemical reactions in analytical chemistry and biological systems.
Journal: RSC Advances
Publication Title: Microfabricated needle for hydrogen peroxide detection
Authors: Shilun Feng, Sandhya Clement, Yonggang Zhu, Ewa M. Goldys and David W. Inglis
Abstract: A microfabricated needle-like probe has been designed and applied for hydrogen peroxide (H2O2) sampling and detection using a commercial, single-step fluorescent H2O2 assay. In this work, droplets of the assay reagent are generated and sent to the needle tip using a mineral-oil carrier fluid. At the needle tip, the sample is drawn into the device through 100 mm long hydrophilic capillaries by negative pressure. The sampled fluid is immediately merged with the assay droplet and carried away to mix and react, producing a sequence of droplets representing the H2O2 concentration as a function of time. We have characterized the assay fluorescence for small variations in the sample volume. With the calibration, we can calculate the concentration of H2O2 in the sampled liquid from the size and intensity of each merged droplet. This is a microfluidic data-logger system for on-site continuous sampling, controlled reaction, signal storage and on-line quantitative detection. It is a useful tool for monitoring dynamic chemical reactions in analytical chemistry and biological applications.
Key words: Microfluidics, probe, H2O2, analytics chemistry
Recent publication by CNBP PhD student Mr Yi Li and team at the University of New South Wales explores the challenges and opportunities of working with CRISPR /Cas for multiplex detection
Journal: Trends in Biotechnology
Authors: Yi Li, Linyang Liu, Guozhen Liu
Abstract: Performing multiplex detection is still an elusive goal for molecular diagnostics. CRISPR/Cas-based biosensing has demonstrated potential for multiplex detection. Instead of being an insurmountable obstacle, CRISPR/Cas multiplexed biosensing is a realistic challenge with some recent successful applications. Strategic considerations are required to fully explore its potential in multiplex diagnostics.
A molecular imprinted polymer biosensing device (developed on stainless steel) that can successfully detect cytokines has been reported by CNBP researchers. Cytokines are proteins secreted by cells that stimulate surrounding cells into specific action and are important to an organism’s immune responses. The finding was reported in the journal ‘Sensors and Actuators B: Chemical’ with the lead author of the publication being CNBP’s Fei Deng based at UNSW Sydney.
Journal: Sensors and Actuators B: Chemical.
Authors: Fei Deng, Ewa M. Goldys, Guozhen Liu.
Abstract: A molecularly imprinted polymer (MIP) based biosensing device on stainless steel (SS) for detection of locally variable concentration of cytokine interleukin-1β (IL-1β) was successfully developed using a sandwich assay scheme. The SS surface was firstly modified with a layer of polydopamine (PDA) followed by the attachment of a layer of poly(ethyleneimine) (PEI) by electrostatic adsorption. Subsequently, the template protein IL-1β was adsorbed on the PEI terminated SS surface due to electrostatic adsorption. A PDA imprinting film was then in-situ synthesized on the surface of the modified SS substrate with incorporated template cytokine. Finally, the template was washed off the SS substrate leaving behind cavities with specific shape and capable of capturing cytokines thus forming a MIP biosensing interface. After exposure to the analyte IL-1β, the MIP biosensing device was incubated with IL-1β detection antibody-modified fluorescent polystyrene beads allowing to determine the amount of captured IL-1β based on fluorescence intensity. The device has been demonstrated to detect IL-1β with low detection limit of 10.2 pg mL−1, and a linear detection range of 25–400 pg mL−1. This MIP biosensing device can be regenerated more than three times with coefficient of variation 2.08%. The device was applied for the detection of IL-1β secreted by rat macrophages, where the good specificity and selectivity were achieved. MIP serves in this device as a superior substitute of antibody with exceptional stability and reusability. The MIP based biosensing technology presented in our work paves a new way for developing a universal and robust sensing platform for the detection of spatially localised small proteins with low physical concentration.
Professor Ewa Goldys, CNBP Deputy Director, UNSW Sydney, in partnership with Associate Professor Shane Grey from the Garvan Institute have received an international grant from JDRC for “Noninvasive assessment of islet cells”.
This project will develop a non-invasive method for real-time monitoring of encapsulated beta cells in vitro and in vivo. This will help assess the fate of implanted cells and define the conditions required to produce high quality insulin-producing cells for implantation and their precursors.
The results will lay the foundations for in vivo assessment of islet transplantation success.