Prof Jeremy Thompson, CNBP Chief Investigator at the University of Adelaide was a panelist at a ‘Science in the Pub’ outreach event where he presented a talk titled ‘Life outside the womb’ at the Rob Roy Hotel, Adelaide, September 7th, 2018.
Year 12 chemistry/biology students from Temple Christian College were given a tour around the Braggs building and CNBP laboratories at the University of Adelaide by Centre PhD student Kathryn Palasis.
As a part of the tour students were shown the chemistry and laser laboratories and were also shown the glass and fibre fabrication facilities to aid understanding of the type of research that is undertaken by CNBP and others in the research space.
CNBP’s Dr Jiawen Li has given a science talk at the College of Optical Science and Engineering, Zhejiang University, China, 7th June, 2018. The talk’s title was ‘Miniaturized multimodal fibre-optic probes for biomedical applications’.
While at the college, Dr Li also visited laboratories specialising in super resolution microscopy, holography and optical coherence tomograpy (OCT). She also shared with undergraduate and master students, her experiences of studying in both the United States and Australia, and provided her perspective on potential career paths for post-doctorate researchers.
CNBP scientists Chris Ashwood (pictured) and Prof Nicki Packer at Macquarie University have shown that sugars with exactly the same chemical composition but slightly different structure break apart differently in their latest publication in the area of mass spectrometry. This work is their first step in automating sugar analysis, to understand the role sugars play in human disease.
Journal: Journal of The American Society for Mass Spectrometry.
Authors: Christopher Ashwood, Chi-Hung Lin, Morten Thaysen-Andersen, Nicolle H. Packer.
Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology. As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments. We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS. By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered. Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions. These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates. Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination. This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.
Macquarie University and CNBP PhD student, Christopher Ashwood, has won a poster prize at the 23rd Annual Lorne Proteomics Symposium.
His poster was about improving reproducibility in the field of glycomics, the study of protein glycosylation.
Over 280 researchers attended the meeting.
Sameera Iqbal, CNBP PhD student at Macquarie University has been awarded a certificate and cash prize for her poster presentation at the Australasian Glycoscience Symposium at the Lorne Proteomics Conference, 2 Feb, 2018.
Her poster detailed the following work –
‘PolySialic Acid (PolySia) is an α2-8-linked sialic acid chain present on cell surfaces in embryonic brains. Changes in polysialylation pattern are reported to be associated with immune defense and inflammation in the CNS. Opioids such as Morphine-3-Glucuronide (M3G) (metabolite of morphine) activates neuroinflammation in a manner parallel to Lipopolysaccharide (LPS), compromising opioid-induced analgesia. In this study, morphine (Morphine-3-glucuronide) was hypothesized to affect the polySia expression in neurons and astrocyte cell lines. It was observed that PolySia expression was significantly increased in neurons following LPS and M3G stimulation.’
Well done Sameera!
Fantastic TV talents (and CNBP researchers) Prof Brant Gibson and Dr Philipp Reineck from RMIT University featured on SCOPE TV for kids, 11th November 2017.
Check them out as they discuss the use of diamond nanoparticles in biophotonics to help shed light on cells and the living body!
The world’s smallest fibre-optic probe that can simultaneously see and sense deeply inside the body (Dr Jiawen Li) and an anti-cancer drug that can be switched ‘on’ and ‘off’ inside the body to help reduce chemotherapy side effects (PhD student Kathryn Palasis). These were the research narratives developed by the two CNBP scientists who attended the ‘Fresh Science’ outreach training program on the 7th-8th November in Adelaide, South Australia.
“I had a great time participating in Fresh Science,” said Kathryn Palasis.
“We had a full day of media training which included practise interviews with journalists from TV, radio and print, who taught us how to best explain our science to the general public. We then had the opportunity to present our work to some very eager and inquisitive school students, and later had to summarise our research to a crowd at the pub in the time it took for a sparkler to burn out! It was a great learning experience and a lot of fun – plus I got to meet some really cool local researchers who are all doing exciting work.”
Dr Jiawen Li also enjoyed the experience. “What I got from the program was the ability to promote my science to the media, knowledge on how to be noticed by journalists and the experience of being interviewed, as well as broader presentation skills aimed at communicating complicated science concepts to a general audience. The two days were extremely rewarding!”
Fresh Science (run by Science in Public) is a national competition helping early-career researchers find, and then share, their stories of discovery. The program takes up-and-coming researchers with no media experience and turns them into spokespeople for science, with a day of media training and a public outreach event in their home state.
Centre Associate Investigator, Dr Kate Fox from RMIT University has participated in the Ecolinc STEM for Women program in Melbourne, Oct 16th, 2017.
Ninety students (Years 9 and 10) attended the event where they got to learn of the experiences from a range of women who work in a variety of STEM related areas. They also heard from education providers about potential career pathways in STEM and listened to the career journeys of successful women in science.
The students were from Upper Yarra, St Albans, Dandenong, Southern Cross Grammar, Bacchus Marsh, Overnewton College, Highview College, Bellarine and Whittlesea.
Aimee Horsfall, CNBP PhD student at the University of Adelaide has been awarded a student Poster Award (of four), with cash prize, for her poster titled “Introduction of a new fluorescent constraint on-resin” at the 6th Modern Solid-Phase Peptide Synthesis Symposium, held at Fraser Island from the 12-14th of October. The symposium is a satellite conference of the 12th Australian Peptide Conference which was held in Noosa from the 15-20th October which Aimee also attended.