CNBP PhD Student Ms Yuan Qi Yeoh enjoyed a two week collaborative visit with Prof Xuefeng Guo’s team at Peking University. Working with Peking University PhD student Xinjiani Chen on a research project involving the molecular dynamics of the secondary structure of a cyclic photoswitchable peptide.
Yuan Qi had the opportunity to participate in the fabrication process of the single-molecule devices. Specifically, they carried out temperature-dependent experiments using their advanced facilities to probe the molecular dynamics of the secondary structure upon photoswitching.
Yuan Qi says that “It was a great opportunity to collaborate with colleagues at Peking University in such high impact research and enjoyed working in their sophisticated research labs”
23 October 2018:
‘Ingenuity’, a public facing event run by the Faculty of Engineering, Computer and Mathematical Sciences (University of Adelaide) was recently held at the Adelaide Convention Centre and CNBP science was represented!
The University event, showcasing final year student projects and achievements, was attended by thousands of school students, industry representatives and members of the general public, with the goal of encouraging and fostering an ongoing interest in STEM related subject areas (science, technology, engineering and maths).
This year saw CNBP PhD student Kathryn Palasis participate at the event, giving two presentations to approximately 300 school students on her research (the design and development of photoswitchable drugs) and describing her time at the University, with the aim of encouraging students to pursue a career in STEM.
“It was fantastic seeing the energy and interest in the room,” said Miss Palasis. “The feedback from staff and students was extremely positive and it was great to share my research and scientific passion with them all.”
“Hopefully we’ll see some of these young scientists studying at the University and then showcasing their own exciting areas of research in the years to come,” she said.
Below – CNBP PhD student Kathryn Palasis delivers her talk.
7 September 2018:
Prof Jeremy Thompson, CNBP Chief Investigator at the University of Adelaide was a panelist at a ‘Science in the Pub’ outreach event where he presented a talk titled ‘Life outside the womb’ at the Rob Roy Hotel, Adelaide, September 7th, 2018.
27 July 2018:
Year 12 chemistry/biology students from Temple Christian College were given a tour around the Braggs building and CNBP laboratories at the University of Adelaide by Centre PhD student Kathryn Palasis.
As a part of the tour students were shown the chemistry and laser laboratories and were also shown the glass and fibre fabrication facilities to aid understanding of the type of research that is undertaken by CNBP and others in the research space.
7 June 2018:
CNBP’s Dr Jiawen Li has given a science talk at the College of Optical Science and Engineering, Zhejiang University, China, 7th June, 2018. The talk’s title was ‘Miniaturized multimodal fibre-optic probes for biomedical applications’.
While at the college, Dr Li also visited laboratories specialising in super resolution microscopy, holography and optical coherence tomograpy (OCT). She also shared with undergraduate and master students, her experiences of studying in both the United States and Australia, and provided her perspective on potential career paths for post-doctorate researchers.
30 March 2018:
CNBP scientists Chris Ashwood (pictured) and Prof Nicki Packer at Macquarie University have shown that sugars with exactly the same chemical composition but slightly different structure break apart differently in their latest publication in the area of mass spectrometry. This work is their first step in automating sugar analysis, to understand the role sugars play in human disease.
Journal: Journal of The American Society for Mass Spectrometry.
Publication title: Discrimination of Isomers of Released N- and O-Glycans Using Diagnostic Product Ions in Negative Ion PGC-LC-ESI-MS/MS.
Authors: Christopher Ashwood, Chi-Hung Lin, Morten Thaysen-Andersen, Nicolle H. Packer.
Profiling cellular protein glycosylation is challenging due to the presence of highly similar glycan structures that play diverse roles in cellular physiology. As the anomericity and the exact linkage type of a single glycosidic bond can influence glycan function, there is a demand for improved and automated methods to confirm detailed structural features and to discriminate between structurally similar isomers, overcoming a significant bottleneck in the analysis of data generated by glycomics experiments. We used porous graphitized carbon-LC-ESI-MS/MS to separate and detect released N- and O-glycan isomers from mammalian model glycoproteins using negative mode resonance activation CID-MS/MS. By interrogating similar fragment spectra from closely related glycan isomers that differ only in arm position and sialyl linkage, product fragment ions for discrimination between these features were discovered. Using the Skyline software, at least two diagnostic fragment ions of high specificity were validated for automated discrimination of sialylation and arm position in N-glycan structures, and sialylation in O-glycan structures, complementing existing structural diagnostic ions. These diagnostic ions were shown to be useful for isomer discrimination using both linear and 3D ion trap mass spectrometers when analyzing complex glycan mixtures from cell lysates. Skyline was found to serve as a useful tool for automated assessment of glycan isomer discrimination. This platform-independent workflow can potentially be extended to automate the characterization and quantitation of other challenging glycan isomers.
2 February 2018:
Macquarie University and CNBP PhD student, Christopher Ashwood, has won a poster prize at the 23rd Annual Lorne Proteomics Symposium.
His poster was about improving reproducibility in the field of glycomics, the study of protein glycosylation.
Over 280 researchers attended the meeting.
2 February 2018:
Sameera Iqbal, CNBP PhD student at Macquarie University has been awarded a certificate and cash prize for her poster presentation at the Australasian Glycoscience Symposium at the Lorne Proteomics Conference, 2 Feb, 2018.
Her poster detailed the following work –
‘PolySialic Acid (PolySia) is an α2-8-linked sialic acid chain present on cell surfaces in embryonic brains. Changes in polysialylation pattern are reported to be associated with immune defense and inflammation in the CNS. Opioids such as Morphine-3-Glucuronide (M3G) (metabolite of morphine) activates neuroinflammation in a manner parallel to Lipopolysaccharide (LPS), compromising opioid-induced analgesia. In this study, morphine (Morphine-3-glucuronide) was hypothesized to affect the polySia expression in neurons and astrocyte cell lines. It was observed that PolySia expression was significantly increased in neurons following LPS and M3G stimulation.’
Well done Sameera!