A novel technique developed by researchers at the ARC Centre of Excellence for Nanoscale BioPhotonics (CNBP) will help shine new light on biological questions by improving the quality and quantity of information that can be extracted in fluorescence microscopy.
The technique, ‘bleaching-assisted multichannel microscopy’ (BAMM) takes a current long-standing weakness of fluorescence microscopy – photobleaching – and turns it into a strength that improves imaging output by up to three times, with no additional hardware required.
Reported in the journal ‘Biomedical Optics Express’ (lead author Dr Antony Orth, CNBP Research Fellow at RMIT University), BAMM will help researchers gain biological insights into the intricate processes taking place within living cells. This includes the interplay between proteins and molecules which have the potential to impact a wide range of health areas from fertility, to pain, to heart disease and more.
Publication authors: Antony Orth, Richik N. Ghosh, Emma R. Wilson, Timothy Doughney, Hannah Brown, Philipp Reineck, Jeremy G. Thompson, and Brant C. Gibson.
Below – This figure shows the information-rich cellular images made possible by using the newly reported BAMM technique. The ‘Original’ image shows cells containing multiple fluorescent targets, all having similar colours. This results in a monochrome image. With BAMM, photobleaching rates are colour coded red, green and blue for visualisation, so that each fluorescently labelled structure can be identified even though the fluorophore’s native colour information was never used.