Monthly Archives: November 2017

Cytokine detection

6 November 2017:

New research from CNBP scientists reports on a cytokine sensor – fabricated on the surface of an optical fibre. Cytokines are molecules that play a critical role in cellular response to infection, inflammation, trauma and disease. Lead author on the paper, published in the journal ‘Biosensors and Bioelectronics’, is Centre PhD student Kaixin Zhang who is based at Macquarie University.

Journal: Biosensors and Bioelectronics.

Publication title: Robust immunosensing system based on biotinstreptavidin coupling for spatially localized femtogram mL−1 level detection of interleukin-6.

Authors: Kaixin Zhang, Guozhen Liu, Ewa M. Goldys.

Abstract: Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids is important in biomedical research and clinical applications. Here, we demonstrate spatially-localised ultrasensitive (femtogram mL−1) level detection of IL-6 in serum and in cell culture media. Our approach is based on a sandwich immunosensor fabricated on the surface of an optical fibre. Firstly, the biotinylated IL-6 capture antibody was immobilized on the fibre surface by biotin-streptavidin coupling. Then the fabricated fibre was used for capturing IL-6 followed by exposure to detection antibody which was labeled with the fluorescent magnetic nanoparticles to report the signal. A linear relationship between IL-6 concentration and the fluorescence signal was obtained in the range from 0.4 pg mL−1 to 400 pg mL−1 of IL-6, with the limit of detection down to 0.1 pg mL−1. In addition, this optical fibre sensor was successfully applied for the localized detection of IL-6 with the spatial resolution of 200 µm and a sample volume of 1 μL. Finally, the performance of the fibre sensor was demonstrated by detection of IL-6 secreted by BV-2 cells with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).

Concordia College students visit CNBP

6 November 2017:

Thirty-one Year 11 students from Concordia College visited CNBP headquarters at the University of Adelaide, 6th November 2017, further strengthening outreach engagement and linkages between the school and Centre researchers.

The students, part of the  International Baccalaureate Science program, enjoyed presentations from CNBP researchers, participated in a Q&A regarding CNBP science, and undertook lab tours with Dr Jiawen Li who did a show and tell with Miniprobes technology. Students were then able to get hands-on with the mini-probe, experimenting with its capabilities on pieces of fruit which mimicked potential use on the human body.

As part of the outreach session – CNBP’s Dr Kyle Dunning talked about her research and its focus on reproductive health, Patrick Capon and Aimee Horsfall presented chemistry and its use in CNBP sensing technology and Dr Georgios Tsiminis  talked about his own physics journey and the sensing work that he is now working on in the meat and dairy space.

Feedback from Joanne Rogers, Head of Science at Concordia College, noted that she thought this outreach session was, “The best yet with CNBP.”

Below – photos from the visit.