The latest paper published by CNBP researchers (lead author Nima Sayyadi, pictured left), reports on a bright red water soluble luminescent molecular probe that was successfully synthesized, with multiple platforms developed for sensitive immunodetection of prostate cancer cells. The probe has immediate potential for sensitive detection of a wide range of proteins and disease-specific cellular antigens.
Journal: Analytical Chemistry.
Publication title: Sensitive time-gated immunoluminescence detection of prostate cancer cells using a TEGylated europium ligand.
Authors: Nima Sayyadi, Irene Justiniano, Russell Edwin Connally, Run Zhang, Bingyang Shi, Liisa Kautto, Arun V Everest-Dass, Jingli Yuan, Bradley John Walsh, Dayong Jin, Robert Drant Willows, James A. Piper, and Nicolle H. Packer.
Abstract: We describe the application of a synthetically developed tetradentate β-diketonate-europium chelate with high quan-tum yield (39%), for sensitive immunodetection of prostate cancer cells (DU145). MIL38 antibody, a mouse monoclonal antibody against Glypican 1, conjugated directly to the chelate via lysine residues, resulted in soluble (hydrophilic) and stable immunoconjugates. Indirect labeling of the antibody by a europium chelated secondary polyclonal antibody and a streptavidin/biotin pair was also performed. All of these bright luminescent conjugates were used to stain DU145 cells, a prostate cancer cell line, using time gated luminescence microscopy for imaging, and their performances were compared to conventional FITC labelling. For all prepared conjugates, the europium chelate in conjunction with a gat-ed auto-synchronous luminescence detector (GALD) completely suppressed the cellular autofluorescence background to allow capture of vivid, high contrast images of immune-stained cancer cells.
The paper is available online.