Proteomics paper examines knee osteoarthritis

Arun215 March 2016:

CNBP researcher Arun  Everest-Dass and CNBP Chief Investigator Prof Nicolle Packer have both co-authored a newly published paper that reports on a new approach, to better study and understand knee osteoarthritis. The paper was published in the journal ‘Proteomics’.

Title: MALDI mass spectrometry imaging of N-glycans on tibial cartilage and subchondral bone proteins in knee osteoarthritis.

Authors: Matthew T Briggs, Julia S Kuliwaba, Dzenita Muratovic, Arun V Everest-Dass, Nicolle H Packer, David M Findlay and Peter Hoffmann.

Abstract: Magnetic Resonance Imaging (MRI) is a non-invasive technique routinely used to investigate pathological changes in knee osteoarthritis (OA) patients. MRI uniquely reveals zones of the most severe change in the subchondral bone (SCB) in OA, called bone marrow lesions (BMLs). BMLs have diagnostic and prognostic significance in OA, but MRI does not provide a molecular understanding of BMLs. Multiple N-glycan structures have been observed to play a pivotal role in the OA disease process. We applied matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) of N-glycans to formalin-fixed paraffin-embedded (FFPE) SCB tissue sections from patients with knee OA, and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was conducted on consecutive sections to structurally characterize and correlate with the N-glycans seen by MALDI-MSI. The application of this novel MALDI-MSI protocol has enabled the first steps to spatially investigate the N-glycome in the SCB of knee OA patients.

The paper can be accessed online.