Monthly Archives: December 2014

CNBP seminar – Macquarie University

cnbplogosquare117 December 2014: Local Event

Visiting Researcher Professor Sergey Deyev delivered a seminar at Macquarie Node entitled: Multifunctional nanoconstructs for bioimaging and therapy.

Summary: Numerous self-assembly systems have been designed, which allow for control of assembly-disassembly with relatively gentle stimuli. These have applications for the construction of “smart” materials for a number of applications (drug delivery, biosensors, etc.).  We address the stability of the barnase−barstar system (BBS) -“glued” assemblies subject to destruction. To this end, we test their behavior under severe protein denaturing conditions such as high temperature and low pH as well as high salt and chaotropic agent (urea and guanidinium hydrochloride) concentrations.

Alternatively, it is desirable to design materials that are hard to disassemble. For example, self-assembled multifunctional theranostic agents are expected to demonstrate significant stability to ensure retention of all functional modules within a single entity to be able to perform all programmed functions e.g., imaging, drug delivery, stimulus-responsiveness. We studied such robust protein-assisted nanoparticle self-assembly systems based on barnase-barstar, streptavidin-biotin, antibody-antigen, and protein A-immunoglobulin interactions. The barnase-barstar pair is particularly advantageous due to its productions by genetic engineering. N- and C-termini of both proteins are not involved in the molecular interface of the proteins within the complex, so they are available for fusions such as those with antibodies, fluorescent proteins, and bacterial toxins, which can be used as additional functional modules of the hybrid protein-particle constructions.

The results on applications of barnase-barstar platform with important types of the nanoparticles, including quantum dots, luminescent nanodiamonds, colloidal gold, magnetic NPs, luminescent upconversion NPs as well as delivery of pseudomonas exotoxin A and radioisotope to the HER2/neu overexpressing human adenocarcinoma cells are presented.

 

 

Nanoparticles, velcro, marbles and a hot glue gun

CNBPat Questacon SciComm event Dec 2014-2

16 December 2014 :Science Communication workshop.
“If you can’t explain it simply, you don’t understand it well enough.” – Albert Einstein.

On December 15-16th, 9 CNBP ECRs from University of Adelaide,  RMIT University, Macquarie University and SAHMRI attended a science communication workshop at the Questacon workshop (Canberra) and were taught the importance of translating their research into lay terms. More specifically, explaining concepts such as the use of nanoparticles for targeted drug therapy to an 8 year old. Its harder than you think and involves the art of scaffolding; i.e. simplifying a concept by using analogies.

The most interactive and fun session of the workshop involved us creating models and physical demonstrations of our research and included us all running around the Questacon workshop to explain current, throwing paper at people as a demonstration of drug delivery, different coloured glow sticks as an analogy for the use of nanoparticles/probes to diagnose pain and the use of lots and lots of velcro and hot glue guns to make “up-scaled” nanoparticles. Think Playschool for scientists.

CNBP were proud to be sponsors of this professional development workshop which was developed by The ARC Centre of Excellence for Electromaterials Science, in partnership with the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology and the ARC Centre of Excellence in Nanoscale BioPhotonics.

The workshop was designed to teach participants how to put their best foot forward in media interviews, community talks and scientific presentations.

Prof Sergey Deyev visits Macquaire Node

15 Dec 20CNBP_candy14 – 15 January 2015: Visitor

Professor Sergey Deyev from the Institute of Bioogranic Chemistry, Moscow will visit the CNBP Macquarie Node between 15 December 2014 and 15 January 2015.

Professor Deyev is visiting Prof Ewa Goldys and our AI Andrei Zvyagin

Biography highlights

Professor Deyev graduated Lomonosov Moscow State University, Department of Chemistry, obtained PhD degree in Molecular Biology, followed by a.Sci. degree in Molecular Biology at the Engelhardt Institute of Molecular Biology.  His primary position is a Deputy Head of Immunology Department at Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow; and also the Corresponding Member of Russian Academy of Sciences highly regarded in the Russian academic system.  His research experience and interests include:

Targeted delivery of nano-agents (nanogold, magnetic nanoparticles, toxins, quantum dots, radioisotopes) to tumors for cancer diagnostics (bioimaging) and therapy, self-assembly of nanoparticles;

Introduction of the recombinant production of a high-affinity protein pair barnase:barstar to enable new immunocytochemistry methods, multivalent complexes production, and targeted drug delivery;

Development of functional homo- and multivalent complexes, such as immunotoxins, photosensitisers, based on recombinant proteins.

Stably transfected fluorescent cancer cell lines for in vivo study of cancer progression and monitoring of treatment were developed.

 

IPAS Pilot Project Grants Awarded

3d-printed-extrusion-july-news-img

15 December 2014: Three Grants awarded to CNBP researchers

CNBP resarchers at the University of Adelaide were successful in obtaining three IPAS pilot project grants totaling $45,000

Characterisation of aquaporin-1 (AQP1) ion channel activity in migrating cancer cells using a novel photoswitchable fluorescent probe, Andrea Yool, Sabrina Heng, Jinxin (Victor) Pei, $15K

Rapid phenotyping of human stem cells sub-populations using optical spectroscopy, Georgios Tsiminis, Erik Schartner, Simon Koblar, Mark Hutchinson, $10K

UV-guiding silica hollow-core fibre for biological sensing, Philip Light, Sabrina Heng, Chris Perrella, Heike Ebendorff-Heidepriem, $15K

Go8 German Research Cooperative Grant

Heike Ebendorff Low Res Edit 018015 December 2014: New grant funding.

Congratulations to CNBP researchers A/Prof Heike Ebendorff-Heidepriem and colleague Dr Jiangbo (Tim) Zhao  for the being awarded funding under the 2015-2016 Group of Eight Australia – Germany Joint Research Co-operation Scheme for the project “Incorporating Pd nanoparticles into fibre for plasmon-enhanced UV-blue photonic devices” ($20k awarded).

This scheme is a joint initiative of the Go8 and the German Academic Exchange Service (DAAD), Germany’s national agency for the support of international academic co-operation. More information about the Scheme.