Visiting Researcher Professor Sergey Deyev delivered a seminar at Macquarie Node entitled: Multifunctional nanoconstructs for bioimaging and therapy.
Summary: Numerous self-assembly systems have been designed, which allow for control of assembly-disassembly with relatively gentle stimuli. These have applications for the construction of “smart” materials for a number of applications (drug delivery, biosensors, etc.). We address the stability of the barnase−barstar system (BBS) -“glued” assemblies subject to destruction. To this end, we test their behavior under severe protein denaturing conditions such as high temperature and low pH as well as high salt and chaotropic agent (urea and guanidinium hydrochloride) concentrations.
Alternatively, it is desirable to design materials that are hard to disassemble. For example, self-assembled multifunctional theranostic agents are expected to demonstrate significant stability to ensure retention of all functional modules within a single entity to be able to perform all programmed functions e.g., imaging, drug delivery, stimulus-responsiveness. We studied such robust protein-assisted nanoparticle self-assembly systems based on barnase-barstar, streptavidin-biotin, antibody-antigen, and protein A-immunoglobulin interactions. The barnase-barstar pair is particularly advantageous due to its productions by genetic engineering. N- and C-termini of both proteins are not involved in the molecular interface of the proteins within the complex, so they are available for fusions such as those with antibodies, fluorescent proteins, and bacterial toxins, which can be used as additional functional modules of the hybrid protein-particle constructions.
The results on applications of barnase-barstar platform with important types of the nanoparticles, including quantum dots, luminescent nanodiamonds, colloidal gold, magnetic NPs, luminescent upconversion NPs as well as delivery of pseudomonas exotoxin A and radioisotope to the HER2/neu overexpressing human adenocarcinoma cells are presented.
15 Dec 2014 – 15 January 2015: Visitor
Professor Sergey Deyev from the Institute of Bioogranic Chemistry, Moscow will visit the CNBP Macquarie Node between 15 December 2014 and 15 January 2015.
Professor Deyev is visiting Prof Ewa Goldys and our AI Andrei Zvyagin
Professor Deyev graduated Lomonosov Moscow State University, Department of Chemistry, obtained PhD degree in Molecular Biology, followed by a.Sci. degree in Molecular Biology at the Engelhardt Institute of Molecular Biology. His primary position is a Deputy Head of Immunology Department at Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow; and also the Corresponding Member of Russian Academy of Sciences highly regarded in the Russian academic system. His research experience and interests include:
Targeted delivery of nano-agents (nanogold, magnetic nanoparticles, toxins, quantum dots, radioisotopes) to tumors for cancer diagnostics (bioimaging) and therapy, self-assembly of nanoparticles;
Introduction of the recombinant production of a high-affinity protein pair barnase:barstar to enable new immunocytochemistry methods, multivalent complexes production, and targeted drug delivery;
Development of functional homo- and multivalent complexes, such as immunotoxins, photosensitisers, based on recombinant proteins.
Stably transfected fluorescent cancer cell lines for in vivo study of cancer progression and monitoring of treatment were developed.
15 December 2014: New member of the RMIT Node.
The CNBP are pleased to welcome Dr Daniel Drumm to the team. Dan will be joining the Measure Team with Prof Andrew Greentree based at RMIT University
15 December 2014: Three Grants awarded to CNBP researchers
CNBP resarchers at the University of Adelaide were successful in obtaining three IPAS pilot project grants totaling $45,000
Characterisation of aquaporin-1 (AQP1) ion channel activity in migrating cancer cells using a novel photoswitchable fluorescent probe, Andrea Yool, Sabrina Heng, Jinxin (Victor) Pei, $15K
Rapid phenotyping of human stem cells sub-populations using optical spectroscopy, Georgios Tsiminis, Erik Schartner, Simon Koblar, Mark Hutchinson, $10K
UV-guiding silica hollow-core fibre for biological sensing, Philip Light, Sabrina Heng, Chris Perrella, Heike Ebendorff-Heidepriem, $15K
Congratulations to CNBP researchers A/Prof Heike Ebendorff-Heidepriem and colleague Dr Jiangbo (Tim) Zhao for the being awarded funding under the 2015-2016 Group of Eight Australia – Germany Joint Research Co-operation Scheme for the project “Incorporating Pd nanoparticles into fibre for plasmon-enhanced UV-blue photonic devices” ($20k awarded).
This scheme is a joint initiative of the Go8 and the German Academic Exchange Service (DAAD), Germany’s national agency for the support of international academic co-operation. More information about the Scheme.
Finishing 2014 on a high note the Macquarie CNBP node organized a CNBP-themed MQ BioFocus Research Conference on 10 December.
An ECR-led Conference Committee comprising Dr Wei Deng, Ms K. Drozdowicz-Tomsia, and Dr Varun Sreenivasan put together an exciting program of talks and posters, covering disciplines from medicine to laser physics.
Rarely have we seen more lively discussions!
The meeting was attended by 70 staff and students from CNBP and Macquarie MQ BioFocus Research Centre led by Professor Ewa Goldys (Deputy Director, CNBP).
CNBP Associate Investigators (pictured above) A/Prof Igor Aharonovich (right) and Andrei Zvyagin (left) have presented their research.
MQ BioFocus Research Centre was established in 2010 and it laid the foundation for the CNBP node at Macquarie.
Today, Senior Director of SPIE, Dr Andrew Brown visited the CNBP node at Macquarie.
SPIE the international society for optics and photonics is the key professional organisation set to promote and develop the light-based science and technology as well as the whole spectrum of their applications.
The Macquarie node showcases their research and facilities.
We also discussed our upcoming CNBP International launch which will take place at Photonics West (BIOS) in San Francisco 7-12 February 2015
BIOS is the world largest and most important annual event in Biophotonics and Biomedical Optics